Gerd Klaus Schneider

Partial coherence may be the real estate of two waves whose relative period undergoes random fluctuations which are not, however, sufficient to make the wave totally incoherent. Lighting with partial coherent lighting generates significantly higher contrast for small object details in comparison to incoherent illumination. Combining this process with a high-resolution zoom lens, the researchers could actually visualize the ultrastructures of tissues at hitherto unattained contrast. Slice through the nucleus of a computer mouse adenocarcinoma cell displaying the nucleolus (NU) and the membrane stations running across the nucleus (NMC); used by X-ray nanotomography.

Recent developments in nanoparticle style have generated new alternatives for nano-biotechnology and nano-medicine. Here we applied cryo-soft X-ray tomography (cryo-SXT) to get comprehensive three-dimensional (3D) files to characterise the interaction of superparamagnetic iron oxide nanoparticles (SPION) with a chest cancer cell range. Prof. Dr. Gerd Schneider becomes a full professur for x-ray microscopy at the Humboldt University Berlin and may be the brain of the HZB party “Microscopy”.

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J Schneider

The brand new microscope offers a high-resolution 3-D image of the complete cell in a single step. This is an edge over electron microscopy, when a 3-D image is certainly assembled out of several thin sections. This can take up to weeks first cell.

  • Since every chemical substance element has certain X-ray absorption edges, X-ray microscopy may be used to determine the precise factors in the components of a sample.
  • Likewise, the cell do not need to be labelled with dyes, unlike in fluorescence microscopy, where simply the labelled structures turn out to be visible.
  • An excellent guy!

In addition, the cell need not come to be labelled with dyes, unlike in fluorescence microscopy, where simply the labelled structures come to be visible. The new X-ray microscope instead exploits the pure contrast between organic and natural material and water to form an image of all cell structures. Dr. Gerd Schneider and his microscopy team at the Institute for Soft Matter and Functional Materials have published their enhancement in Nature Procedures. Achieving the highest possible quality in X-ray microscopy calls for high-reliability lenses to target the X-ray beams.

The professorship is associated with heading the workgroup “X-ray microscopy” at the Helmholtz-Zentrum Berlin für Materialien und Energie. With his party, the internationally recognised expert is developing innovative methods and programs for X-ray microscopy, which delivers critical information for most scientific disciplines – from material and power research to the life sciences.

In the chat, we present the new TXM and decided on applications. The transmission X-ray microscope’s 3D PSF exhibits an axial asymmetry that can’t be discussed by an incoherent imaging type for either a lens or zone plate, with or without spherical aberration.

In addition to developing X-ray microscopes, Gerd Schneider’s workgroup has contributed drastically to the progression of these lenses, referred to as Fresnel area plates. Given such 3D X-ray lenses and contemporary synchrotron resources like BESSY II, great contributions can be manufactured towards answering numerous scientific queries, from the basics of structural biology to research into modern strength storage solutions. We developed a new full-field transmitting X-ray microscope (TXM) for automated cryo-tomography and spectroscopy. The system operates at the BESSY undulator U41 at a focusing spherical grating monochromator beamline, which gives a power resolution up to 104.

dr gerd schneider

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